6MQK
Crystal Structure of GTPase Domain of Human Septin 12 in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.960, 69.250, 88.230 |
| Unit cell angles | 75.45, 89.46, 76.43 |
Refinement procedure
| Resolution | 45.586 - 2.190 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mq9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.798 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.590 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.108 | |
| Rpim | 0.068 | 0.607 |
| Number of reflections | 52030 | 3910 |
| <I/σ(I)> | 6.9 | 1.3 |
| Completeness [%] | 96.7 | 96.3 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.995 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.1 M bicine/Trizma base pH 8.5, 30mM magnesium chloride, 30mM calcium chloride |
