6MQJ

Crystal Structure of the All-Trans Retinal bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121Q mutant of Human Cellular Retinoic Acid Binding Protein II Irradiated with 532 nm Laser for 10 minutes at 2.1 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97626
Spacegroup name	P 31 2 1
Unit cell lengths	59.238, 59.238, 99.393
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.587 - 2.125
R-factor	0.1942
Rwork	0.189
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yfp
RMSD bond length	0.007
RMSD bond angle	0.933
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.587	2.201
High resolution limit [Å]	2.125	2.125
Rmerge	0.060	0.515
Rmeas		0.685
Number of reflections	11843	1167
<I/σ(I)>	19.3
Completeness [%]	99.7	98.9
Redundancy	8.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG3350, DL-malic acid