6MQG
Crystal structure of KRAS V14I-GDP demonstrating open switch 1 conformation - Form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 77.751, 77.751, 55.935 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.850 - 1.500 |
| R-factor | 0.1567 |
| Rwork | 0.155 |
| R-free | 0.19450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4obe |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.653 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14rc3_3199) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rpim | 0.024 | 0.255 |
| Number of reflections | 31559 | 1541 |
| <I/σ(I)> | 38 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.5 | 14.5 |
| CC(1/2) | 0.980 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 1.8 M Na/K Phosphate, pH 8.2 |
