6MQB
Crystal Structure of GTPase Domain of Human Septin 12 in complex with GMPPNP in Space Group C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.020, 91.860, 151.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 2.120 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mq9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.135 | 1.042 |
| Rpim | 0.063 | 0.480 |
| Number of reflections | 16639 | 6492 |
| <I/σ(I)> | 7.9 | 1.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.994 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 10% w/v PEG 8000, 20% v/v ethylene glycol, 0.1 M MES/imidazole pH 6.5 and 20mM of each: sodium formate, ammonium acetate, trisodium citrate, sodium potassium l-tartrate, sodium oxamate |
