6MOW

Crystal Structure of the apo R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121Q mutant of Human Cellular Retinoic Acid Binding Protein II at 2.3 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97626
Spacegroup name	P 31 2 1
Unit cell lengths	57.561, 57.561, 102.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.656 - 2.354
R-factor	0.1999
Rwork	0.193
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yfp
RMSD bond length	0.007
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.660	2.439
High resolution limit [Å]	2.354	2.354
Rmerge	0.069	0.580
Rmeas	0.101	0.778
Number of reflections	8421	742
<I/σ(I)>	32.3
Completeness [%]	98.3	88.12
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG3350, Sodium Malonate