6MOS
Structure of thioredoxin 1 from the thermophilic eubacterium Thermosipho africanus TCF52B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.454, 41.023, 55.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.057 - 1.800 |
| R-factor | 0.212317242433 |
| Rwork | 0.211 |
| R-free | 0.24563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.843 |
| Data scaling software | SCALA |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.020 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 8989 | 1198 |
| <I/σ(I)> | 28.2 | 7.3 |
| Completeness [%] | 99.1 | 95 |
| Redundancy | 6.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 289 | 0.2 M MgCl2 , 0.1 M bis-tris pH 6.5, 25%(w/v) PEG 3350 |
