6MOI

Dimeric DARPin C_angle_R5 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	P 65 2 2
Unit cell lengths	112.748, 112.748, 280.793
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.821 - 2.065
R-factor	0.2164
Rwork	0.214
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model; 1ERN
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.821	2.421
High resolution limit [Å]	2.065	2.065
Rmerge	0.466	3.143
Rpim	0.080	6.339
Number of reflections	29498	1475
<I/σ(I)>	9.4	1.6
Completeness [%]	45.0	1.59
Redundancy	37.3	25.2
CC(1/2)	1.000	0.151

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.3 M magnesium sulfate, 0.1 M bis-tris propane pH 7, 22.5% PurePEGs cocktail (Anatrace), 30% ethylene glycol cryoprotectant