6MNS

Rhesus macaque anti-HIV V3 antibody DH753 with gp120 V3 ZAM18 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2017-06-06
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	46.920, 138.315, 71.678
Unit cell angles	90.00, 91.55, 90.00

Refinement procedure

Resolution	38.772 - 2.700
R-factor	0.1769
Rwork	0.170
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Previously solved DH753 structure (also in submission)
RMSD bond length	0.009
RMSD bond angle	1.245
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rpim	0.055	0.278
Number of reflections	25304	1299
<I/σ(I)>	17.6	3
Completeness [%]	99.5	100
Redundancy	4	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium dihydrogen phosphate, 20% PEG 3,350