6MM8
Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.643, 67.860, 58.529 |
| Unit cell angles | 90.00, 92.22, 90.00 |
Refinement procedure
| Resolution | 33.950 - 1.850 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mm7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 4.230 | 1.850 |
| Rmerge | 0.063 | 0.039 | 0.578 |
| Rmeas | 0.074 | 0.046 | 0.714 |
| Rpim | 0.038 | 0.023 | 0.409 |
| Total number of observations | 219079 | ||
| Number of reflections | 58151 | 5034 | 4089 |
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 96.6 | 98.2 | 81.7 |
| Redundancy | 3.8 | 3.8 | 2.9 |
| CC(1/2) | 0.995 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.15 M HEPES pH 7.5, 15% (w/v) PEG 20k, and 25% (v/v) ethylene glycol |
