6MLX
Crystal structure of T. pallidum Leucine Rich Repeat protein (TpLRR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-05 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9202 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 104.079, 104.079, 185.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.308 - 2.000 |
R-factor | 0.2366 |
Rwork | 0.234 |
R-free | 0.28150 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.927 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.900 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 67107 | |
<I/σ(I)> | 15.1 | |
Completeness [%] | 96.2 | |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Bis-Tris HCl, pH 6.5 and 25% PEG 3350 |