6MLT

Crystal structure of the V. cholerae biofilm matrix protein Bap1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.25414
Spacegroup name	P 41 21 2
Unit cell lengths	70.951, 70.951, 303.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 1.900
R-factor	0.1617
Rwork	0.161
R-free	0.17620
Structure solution method	SIRAS
RMSD bond length	0.004
RMSD bond angle	0.641
Data reduction software	XDS (20170615)
Data scaling software	Aimless (0.5.32)
Phasing software	AutoSol (1.11.1)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.640	47.640	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.114	0.070	0.639
Rmeas	0.120	0.073	0.738
Rpim	0.034	0.021	0.356
Number of reflections	62568	710	3772
<I/σ(I)>	13.8
Completeness [%]	99.7	99.2	96.2
Redundancy	11.1	11.2	3.8
CC(1/2)	0.998	0.997	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1M sodium citrate, 10% PEG 3350