6MLC

PHD6 domain of MLL3 in complex with histone H4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 6
Unit cell lengths	85.939, 85.939, 98.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.000 - 1.800
R-factor	0.1952
Rwork	0.194
R-free	0.21600
RMSD bond length	0.010
RMSD bond angle	1.350
Data reduction software	HKL-3000
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.010	42.970	1.830
High resolution limit [Å]	1.800	8.990	1.800
Rmerge	0.098	0.039	1.357
Rmeas	0.103	0.041	1.435
Rpim	0.032	0.013	0.463
Number of reflections	38485	326	2274
<I/σ(I)>	18.7
Completeness [%]	100.0	99.1	99.5
Redundancy	10	9.9	9.2
CC(1/2)	0.999	0.999	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	2 M Na-Form, 0.1 M Tris, pH 8.5