6ML3

ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	46.297, 68.757, 50.010
Unit cell angles	90.00, 93.23, 90.00

Refinement procedure

Resolution	38.361 - 1.683
R-factor	0.2263
Rwork	0.225
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ml2
RMSD bond length	0.003
RMSD bond angle	0.525
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.361	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.099	0.358
Rpim	0.039	0.188
Number of reflections	48340	634
<I/σ(I)>	14.6	2.6
Completeness [%]	73.9	18
Redundancy	5.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	292	20% PEG3350, 0.2 M sodium malonate, pH 5.0