6MKX
Crystal structure of the periplasmic Lysine-, Arginine-, Ornithine-binding protein (LAO) R77A mutant from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.001, 76.750, 86.162 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.081 - 2.284 |
R-factor | 0.1962 |
Rwork | 0.191 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lao |
RMSD bond length | 0.010 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.284 | 2.284 |
Rmerge | 0.109 | 0.294 |
Number of reflections | 11886 | 1009 |
<I/σ(I)> | 24.8 | 3.9 |
Completeness [%] | 99.6 | 91.4 |
Redundancy | 7.2 | 2.4 |
CC(1/2) | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.2 M Sodium acetate trihydrate 0.1 M Sodium cacodylate trihydrate 30% w/v Polyethylene glycol 8,000 |