6MKV
Crystal structure of Retinal-bound holo Q108K:K40L:T51W domain-swapped dimer of human cellular retinol binding protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-18 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.332, 63.137, 118.218 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 39.167 - 2.107 |
| R-factor | 0.2045 |
| Rwork | 0.200 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.170 | 2.140 |
| High resolution limit [Å] | 2.107 | 2.107 |
| Rmerge | 0.090 | 0.530 |
| Rmeas | 0.109 | 0.660 |
| Rpim | 0.060 | |
| Number of reflections | 29781 | 2740 |
| <I/σ(I)> | 14.4 | 1.7 |
| Completeness [%] | 96.5 | 91.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, Sodium acetate, Ammonium acetate |
