6MKG
Crystal structure of penicillin binding protein 5 (PBP5) from Enterococcus faecium in the benzylpenicilin-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 192.315, 192.315, 156.094 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.020 - 2.940 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PBP5 APO OPEN |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.453 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.020 | 3.060 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.242 | 2.673 |
| Number of reflections | 36859 | |
| <I/σ(I)> | 9.2 | 1.7 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 0.1 M trisodium citrate, 2M ammonium sulfate |
