6MJE

Structure of Candida glabrata Csm1: S. cerevisiae Dsn1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.127
Spacegroup name	P 1 21 1
Unit cell lengths	44.368, 203.755, 44.259
Unit cell angles	90.00, 90.36, 90.00

Refinement procedure

Resolution	43.351 - 2.500
R-factor	0.2426
Rwork	0.240
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4r
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.199	0.793
Rmeas	0.219	0.938
Rpim	0.091	0.488
Number of reflections	26122	1446
<I/σ(I)>	18.2
Completeness [%]	96.0
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, 25% PEG3350, cryoprotect with additional 20% PEG400