6MJ8

Structure of Candida glabrata Csm1:Mam1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.124, 58.639, 109.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.915 - 3.030
R-factor	0.272
Rwork	0.271
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4r
RMSD bond length	0.005
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	3.200
High resolution limit [Å]	3.030	3.030
Rmerge	0.308	1.651
Rmeas	0.358	1.919
Rpim	0.136	0.714
Number of reflections	6723	958
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES, pH 6.5, 0.6 M sodium chloride, 20% PEG4000, cryoprotectant: 20% PEG400