6MJ7

Crystal structure of p62 ZZ domain in complex with free arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-10-12
Detector	RDI CMOS_8M
Wavelength(s)	1.278
Spacegroup name	C 1 2 1
Unit cell lengths	44.971, 32.269, 34.941
Unit cell angles	90.00, 122.16, 90.00

Refinement procedure

Resolution	29.579 - 1.412
R-factor	0.1386
Rwork	0.136
R-free	0.16460
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.410	1.410
Rmerge	0.063
Number of reflections	107631
<I/σ(I)>	83.2
Completeness [%]	98.4
Redundancy	13.2
CC(1/2)	0.985	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bicine (pH 9.0), 14% PEG 20K, 4% 1,4-Dioxane