6MJ1
Crystal structure of RefZ (regulator of FtsZ) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-07-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.021, 100.021, 100.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.745 - 2.600 |
| R-factor | 0.2236 |
| Rwork | 0.222 |
| R-free | 0.25360 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.082 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.113 | 0.793 |
| Number of reflections | 16237 | 714 |
| <I/σ(I)> | 11.59 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.59 | 14.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 10% ethanol (v/v), 0.1 M imidazole [pH 8.0], and 0.2 M MgCl2 |
