6MIW

WWE domain of human HUWE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-23
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.134, 60.134, 53.288
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.090 - 2.000
R-factor	0.1814
Rwork	0.180
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FFAS model based on PDB entry 1ujr
RMSD bond length	0.013
RMSD bond angle	1.715
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.070	30.070	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.093	0.023	1.073
Rmeas	0.098	0.024	1.130
Rpim	0.030	0.008	0.352
Total number of observations	81684	875	5967
Number of reflections	7843
<I/σ(I)>	22.4	81.6	2.4
Completeness [%]	100.0	97.9	100
Redundancy	10.4	8.3	10.3
CC(1/2)	0.999	1.000	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Molecular Dimensions Morpheus HT condition D3