6MIC
Crystal Structure of the C-terminal half of the Vibrio cholerae minor pilin TcpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 63.290, 63.290, 236.291 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.382 - 1.531 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qs4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.991 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.800 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.028 | 0.091 |
| Rmeas | 0.033 | 0.105 |
| Rpim | 0.016 | 0.053 |
| Number of reflections | 26889 | 1366 |
| <I/σ(I)> | 26.9 | 12.4 |
| Completeness [%] | 96.1 | 99.4 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.999 | 0.989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | MPD, sodium chloride, sodium acetate |
