6MIC

Crystal Structure of the C-terminal half of the Vibrio cholerae minor pilin TcpB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	63.290, 63.290, 236.291
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.382 - 1.531
R-factor	0.1794
Rwork	0.178
R-free	0.19820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qs4
RMSD bond length	0.008
RMSD bond angle	0.991
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.800	1.560
High resolution limit [Å]	1.530	1.530
Rmerge	0.028	0.091
Rmeas	0.033	0.105
Rpim	0.016	0.053
Number of reflections	26889	1366
<I/σ(I)>	26.9	12.4
Completeness [%]	96.1	99.4
Redundancy	3.8	3.8
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	MPD, sodium chloride, sodium acetate