6MI4

Structure of the I65M mutant of NEMO(51-112) with N- and C-terminal coiled-coil adaptors.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979329
Spacegroup name	P 1 21 1
Unit cell lengths	64.407, 34.124, 77.416
Unit cell angles	90.00, 113.89, 90.00

Refinement procedure

Resolution	38.286 - 2.500
R-factor	0.2542
Rwork	0.252
R-free	0.29480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3brv
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.286	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.124	0.770
Rmeas	0.135	0.851
Rpim	0.054	0.354
Number of reflections	10941	1068
<I/σ(I)>	11.64	2.38
Completeness [%]	98.4	97.42
Redundancy	6.5	6
CC(1/2)	0.998	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.1 M Tris pH 8.0, 5.8% PGA-LM, 5.45% PEG 20,000 mother liquor in MRC2 96 well sitting drop plates.