6MI4
Structure of the I65M mutant of NEMO(51-112) with N- and C-terminal coiled-coil adaptors.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979329 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.407, 34.124, 77.416 |
| Unit cell angles | 90.00, 113.89, 90.00 |
Refinement procedure
| Resolution | 38.286 - 2.500 |
| R-factor | 0.2542 |
| Rwork | 0.252 |
| R-free | 0.29480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3brv |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.286 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.124 | 0.770 |
| Rmeas | 0.135 | 0.851 |
| Rpim | 0.054 | 0.354 |
| Number of reflections | 10941 | 1068 |
| <I/σ(I)> | 11.64 | 2.38 |
| Completeness [%] | 98.4 | 97.42 |
| Redundancy | 6.5 | 6 |
| CC(1/2) | 0.998 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris pH 8.0, 5.8% PGA-LM, 5.45% PEG 20,000 mother liquor in MRC2 96 well sitting drop plates. |
