6MI3
Structure of NEMO(51-112) with N- and C-terminal coiled-coil adaptors.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.919774 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.480, 33.620, 76.870 |
Unit cell angles | 90.00, 115.04, 90.00 |
Refinement procedure
Resolution | 37.754 - 1.783 |
R-factor | 0.2441 |
Rwork | 0.243 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dmd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.141 |
Data reduction software | XDS |
Data scaling software | STARANISO (v3.000) |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2849) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.754 | 1.847 |
High resolution limit [Å] | 1.783 | 1.783 |
Rmerge | 0.080 | 5.483 |
Rmeas | 0.096 | 6.493 |
Rpim | 0.052 | 3.444 |
Number of reflections | 28586 | 431 |
<I/σ(I)> | 5.71 | 0.24 |
Completeness [%] | 67.5 | 15.12 |
Redundancy | 3.3 | 3.4 |
CC(1/2) | 0.997 | 0.089 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris pH 8.0, 5.8% PGA-LM, 5.45% PEG 20,000 |