6MHT
TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND DNA CONTAINING 4'-THIO-2'DEOXYCYTIDINE AT THE TARGET
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 99.860, 99.860, 325.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.050 |
| R-factor | 0.186 * |
| Rwork | 0.186 |
| R-free | 0.23100 |
| Structure solution method | OTHER |
| Starting model (for MR) | 5mht |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.078 |
| Number of reflections | 32880 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 98.0 |
| Redundancy | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 16 * | O'Gara, M., (1996) J. Mol. Biol., 261, 634. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | citrate | ||
| 3 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | protein | 10 (mg/ml) | ||
| 2 | 1 | ammonium sulfate | 1.4-1.8 (M) | ||
| 3 | 1 | 1 | citrate | 50 (mM) | |
| 4 | 1 | 2 | ammonium sulfate | 1.4-1.8 (M) | |
| 5 | 1 | 2 | citrate | 50 (mM) |
