6MHH

Proteus mirabilis ScsC linker (residues 39-49) deletion and N6K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-06
Detector	RIGAKU SATURN 944
Wavelength(s)	1.541870
Spacegroup name	H 3 2
Unit cell lengths	64.040, 64.040, 299.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.986 - 2.083
R-factor	0.1759
Rwork	0.174
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4XVW (chain A residues 47-224)
RMSD bond length	0.003
RMSD bond angle	0.687
Data reduction software	iMOSFLM (7.1.3)
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.990	24.990	2.140
High resolution limit [Å]	2.080	9.080	2.080
Rmerge	0.059	0.061	0.140
Rmeas	0.063	0.065	0.150
Rpim	0.020	0.022	0.053
Number of reflections	14715	207	1135
<I/σ(I)>	27.2
Completeness [%]	99.9	94.8	100
Redundancy	10.1	8.8	7.8
CC(1/2)	0.998	0.996	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7, 32% v/v Jeffamine M-600