6MH4

Crystal Structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Staphylococcus schleiferi, Apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 31 1 2
Unit cell lengths	71.368, 71.368, 318.815
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.380 - 2.150
R-factor	0.1991
Rwork	0.197
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.061
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.380	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	50897	5069
<I/σ(I)>	33.9
Completeness [%]	99.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	2000 mM ammonium sulfate, 100 mM sodium citrate/ citric acid