6MGN

mouse Id1 (51-104) - human hE47 (348-399) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.92
Spacegroup name	P 42 21 2
Unit cell lengths	54.962, 54.962, 81.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.432 - 1.901
R-factor	0.1861
Rwork	0.183
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5T9O
RMSD bond length	0.007
RMSD bond angle	0.714
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rpim	0.026
Number of reflections	10249
<I/σ(I)>	29.3
Completeness [%]	98.7
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1 M potassium phosphate (pH 6.0), 0.25 M NaCl, 22.5% PEG8000.