6MFP

Crystal Structure of the RV305 C1-C2 specific ADCC potent antibody DH677.3 Fab in complex with HIV-1 clade A/E gp120 and M48U1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	63.487, 80.093, 88.688
Unit cell angles	84.82, 82.33, 82.24

Refinement procedure

Resolution	39.600 - 3.000
R-factor	0.217
Rwork	0.214
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgt 6mfj
RMSD bond length	0.006
RMSD bond angle	1.451
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.092	0.442
Number of reflections	30469	1547
<I/σ(I)>	10.2
Completeness [%]	89.1	90.7
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	294	25% PEG 4000 0.1 M MES pH 5.5