6MFC

GphF GNAT-like decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	C 2 2 21
Unit cell lengths	138.334, 145.700, 78.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.962 - 2.589
R-factor	0.236
Rwork	0.234
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ree
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.962	43.962	2.750
High resolution limit [Å]	2.589	7.670	2.590
Rmerge	0.184	0.047	2.998
Rmeas	0.192	0.049	3.114
Number of reflections	24892	1046	3893
<I/σ(I)>	11.73	46.62	0.96
Completeness [%]	99.7	99.2	98.3
Redundancy	13.146	12.068	13.593
CC(1/2)	0.998	0.999	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30-35% PEG 3350, 0.23-0.3 M Ammonium Acetate, 0.1 M BisTris HCl pH 5.5