6MF4

Crystal structure of the polo-box domain of Cdc5 from budding yeast.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-29
Detector	RAYONIX MX300HE
Wavelength(s)	1.28341
Spacegroup name	P 21 21 21
Unit cell lengths	51.080, 65.950, 96.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.060 - 1.800
R-factor	0.181
Rwork	0.181
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4o
RMSD bond length	0.004
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	30819	2611
<I/σ(I)>	12.58
Completeness [%]	100.0
Redundancy	4
CC(1/2)	0.997	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM Bis-Tris pH 8.5, 20 % PEG3350, 200 mM sodium formate.