6MDH
X-ray crystal structure of ISG15 from Myotis davidii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-08 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 66.546, 68.426, 72.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.269 - 1.370 |
R-factor | 0.1625 |
Rwork | 0.161 |
R-free | 0.19560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tla |
RMSD bond length | 0.014 |
RMSD bond angle | 1.389 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmeas | 0.058 | 0.641 |
Number of reflections | 34845 | |
<I/σ(I)> | 33.8 | 2.92 |
Completeness [%] | 99.1 | |
Redundancy | 9.8 | |
CC(1/2) | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | citric acid, PEG 6000, Benzamidine hydrochloride |