6MCZ
Crystal Structure of Human PPARgamma Ligand Binding Domain in Complex with Arachidonic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.040, 62.160, 118.960 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 44.970 - 2.100 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vv2 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.970 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.039 | 0.429 |
| Number of reflections | 74191 | |
| <I/σ(I)> | 10.06 | 1.65 |
| Completeness [%] | 98.4 | 95.47 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1M MOPS, pH 7.4 0.8M Sodium Citrate |
