6MBZ

Structure of Transcription Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	132.380, 141.400, 72.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.700 - 3.210
R-factor	0.266
Rwork	0.265
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y1u
RMSD bond length	0.004
RMSD bond angle	0.793
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.700	70.700	3.290
High resolution limit [Å]	3.210	14.360	3.210
Rmerge	0.105	0.032	1.152
Number of reflections	23029
<I/σ(I)>	13.79
Completeness [%]	100.0	98.9	100
Redundancy	6.6	5.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000