6MBC
Human Bfl-1 in complex with the designed peptide dF4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.466, 42.905, 46.666 |
| Unit cell angles | 90.00, 114.21, 90.00 |
Refinement procedure
| Resolution | 42.563 - 1.752 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.21210 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.068 | 0.061 | 0.339 |
| Rmeas | 0.078 | 0.069 | 0.430 |
| Rpim | 0.036 | 0.031 | 0.259 |
| Number of reflections | 15078 | 821 | 565 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 94.8 | 97.7 | 70.7 |
| Redundancy | 4.1 | 4.5 | 1.9 |
| CC(1/2) | 0.996 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1.8 - 2.0 M NH4SO4, 50 mM MES pH 6.5 |
