6MB5

AAC-IIIb binary with NEOMYCIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	106.120, 106.120, 68.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.951 - 2.200
R-factor	0.2454
Rwork	0.245
R-free	0.26080
Data reduction software	MOSFLM
Data scaling software	Aimless (0.6.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.951	45.950	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.091	0.048	0.534
Rmeas	0.107	0.057	0.634
Rpim	0.055	0.029	0.334
Number of reflections	22720	368	1933
<I/σ(I)>	7.5
Completeness [%]	98.7	98.9	97.8
Redundancy	3.5	3.5	3.3
CC(1/2)	0.995	0.995	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	283	1-3%PEG 4000, 20-25% isopropanol,0.1M HEPES, pH=7.5