6M9Z

X-ray Structure of Branchiostoma floridae fluorescent protein lanFP6G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	81.919, 131.382, 55.076
Unit cell angles	90.00, 116.41, 90.00

Refinement procedure

Resolution	28.000 - 1.200
R-factor	0.128
Rwork	0.128
R-free	0.14600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hvf
RMSD bond length	0.012
RMSD bond angle	1.853
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	30.000	1.240
High resolution limit [Å]	1.200	2.590	1.200
Rmerge	0.046	0.032	0.532
Rmeas	0.053	0.037	0.630
Rpim	0.027	0.019	0.334
Number of reflections	153983	16154	13697
<I/σ(I)>	12.4
Completeness [%]	95.3	99.1	85
Redundancy	3.8	3.9	3.4
CC(1/2)		0.998	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M Na acetate pH 7.0, 12% PEG 3350