6M9Y
X-ray Structure of Branchiostoma floridae fluorescent protein lanFP6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.726, 98.169, 55.145 |
| Unit cell angles | 90.00, 108.07, 90.00 |
Refinement procedure
| Resolution | 28.030 - 1.350 |
| R-factor | 0.1333 |
| Rwork | 0.133 |
| R-free | 0.16880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hvf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.723 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.044 | 0.034 | 0.537 |
| Rmeas | 0.053 | 0.040 | 0.684 |
| Rpim | 0.029 | 0.021 | 0.418 |
| Total number of observations | 284216 | ||
| Number of reflections | 116582 | 12072 | 11220 |
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 93.7 | 95.9 | 90.5 |
| Redundancy | 2.4 | 2.8 | 2.4 |
| CC(1/2) | 0.981 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M Ammonium Citrate pH 7.0, 20% PEG 3350 |
