6M97
Crystal structure of the high-affinity copper transporter Ctr1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.2546 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 73.850, 73.850, 410.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.030 |
| R-factor | 0.2854 |
| Rwork | 0.280 |
| R-free | 0.33231 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.121 | 1.578 |
| Rpim | 0.057 | 0.732 |
| Number of reflections | 8991 | 890 |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM zinc acetate, 50 mM sodium cacodylate pH 5.9, and 26% PEG 400. |
