6M94

Monophosphorylated pSer33 b-Catenin peptide bound to b-TrCP/Skp1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-25
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 31
Unit cell lengths	82.370, 82.370, 112.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.264 - 2.700
R-factor	0.2043
Rwork	0.203
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.264	44.264	2.770
High resolution limit [Å]	2.700	12.080	2.700
Rmerge	0.065	0.043	1.885
Rmeas	0.074	0.050	2.159
Number of reflections	23440	232	1683
<I/σ(I)>	12.38	29.31	0.83
Completeness [%]	99.7	88.2	99.1
Redundancy	4.512	4.086	4.097
CC(1/2)	0.997	0.993	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% PEG 4000 0.1M BTP pH5.5