6M8M
PA14 sugar-binding domain from RTX adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2015-09-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 32.575, 61.309, 79.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.550 - 1.200 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j6y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3120) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 1.241 |
| High resolution limit [Å] | 1.198 | 1.198 |
| Number of reflections | 50698 | |
| <I/σ(I)> | 27.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | Potassium thiocyanate, Bis Tris Propane (pH 7.5), PEG 3350, Glucose |
