6M7M

rac-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21/c 1
Unit cell lengths	15.230, 9.290, 21.060
Unit cell angles	90.00, 108.23, 90.00

Refinement procedure

Resolution	14.466 - 1.101
R-factor	0.2023
Rwork	0.199
R-free	0.23630
RMSD bond length	0.007
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	14.466	14.466	1.130
High resolution limit [Å]	1.100	4.920	1.100
Rmerge	0.076	0.042	0.193
Rmeas	0.094	0.057	0.247
Number of reflections	2097	23	130
<I/σ(I)>	6.99	13.3	2.75
Completeness [%]	94.4	92	84.4
Redundancy	2.852	2.826	2.392
CC(1/2)	0.996	0.998	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M imidazole pH 8.0, 10% (w/v) of PEG 8000