6M74

Crystal structure of Enterococcus hirae L-lactate oxidase M207L in complex with D-lactate form ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	138.130, 138.130, 127.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.540 - 1.520
R-factor	0.1287
Rwork	0.128
R-free	0.14440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m73
RMSD bond length	0.006
RMSD bond angle	1.387
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.540	19.540	1.560
High resolution limit [Å]	1.520	6.800	1.520
Rmerge	0.061	0.034	0.703
Rmeas	0.063	0.035	0.731
Total number of observations	1230530
Number of reflections	93906	1129	6875
<I/σ(I)>	26.96	66.52	4.36
Completeness [%]	99.9	94.8	99.9
Redundancy	13.104	12.805	13.078
CC(1/2)	0.999	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Tris, PEG MME