6M6V
Crystal structure the toxin-antitoxin MntA-HepT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97982 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 56.560, 225.700, 52.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 112.850 - 3.080 |
R-factor | 0.2231 |
Rwork | 0.221 |
R-free | 0.27690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yep |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 112.850 | 3.160 |
High resolution limit [Å] | 3.080 | 3.080 |
Number of reflections | 13153 | 4214 |
<I/σ(I)> | 10.2 | |
Completeness [%] | 99.3 | |
Redundancy | 12.5 | |
CC(1/2) | 0.997 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10 % (w/v) PEG 3350, 0.1M Magnesium acetate, 0.1M HEPS pH 7.0 |