6M6R

Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 8-mer ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2819
Spacegroup name	P 43 21 2
Unit cell lengths	56.843, 56.843, 131.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.036 - 1.890
R-factor	0.1797
Rwork	0.178
R-free	0.22130
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.991
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.040	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.142	1.715
Rmeas	0.158	1.950
Number of reflections	17289	984
<I/σ(I)>	12	1.7
Completeness [%]	96.3	88.4
Redundancy	9.2	7.5
CC(1/2)	0.996	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.1 M Tris pH 8.5, 25% w/v polyethylene glycol 3350