6M63

Crystal structure of a cAMP sensor G-Flamp1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-13
Detector	RAYONIX MX-300
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	87.836, 94.688, 109.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.550 - 2.250
R-factor	0.1804
Rwork	0.178
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3evp 3clp
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.150	0.062	1.011
Rmeas	0.155	0.064	1.050
Rpim	0.041	0.018	0.282
Total number of observations	639301
Number of reflections	44052	2398	2142
<I/σ(I)>	8.3
Completeness [%]	100.0	100	100
Redundancy	14.5	13.6	13.8
CC(1/2)		0.995	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Imidazole, PEG 400