6M5Y

Structure of human galectin-1 tandem-repeat mutant with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-04
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	67.380, 95.640, 37.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.870 - 1.380
R-factor	0.1851
Rwork	0.183
R-free	0.21902
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Model structure obtained using SWISS-MODEL and 1GZW
RMSD bond length	0.018
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.870	1.416
High resolution limit [Å]	1.380	1.380
Rmerge	0.050
Number of reflections	50739	3679
<I/σ(I)>	16.08
Completeness [%]	99.6
Redundancy	6.21
CC(1/2)	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20%(w/v) PEG3350, 200 mM Ammonium citrate dibasic