6M5J

U shaped head to head four-way junction in d(TTCTGCTGCTGAA/TTCTGCAGCTGAA) sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-26
Detector	RAYONIX MX300HE
Wavelength(s)	0.99984
Spacegroup name	P 1 21 1
Unit cell lengths	57.630, 62.086, 30.747
Unit cell angles	90.00, 121.84, 90.00

Refinement procedure

Resolution	19.221 - 1.650
R-factor	0.1876
Rwork	0.187
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m4t
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.700
High resolution limit [Å]	1.640	3.530	1.640
Rmerge	0.045	0.029	0.230
Rmeas	0.053	0.034	0.279
Rpim	0.028	0.018	0.156
Total number of observations	37937
Number of reflections	11117	1126	1038
<I/σ(I)>	21.8
Completeness [%]	97.8	95.8	91.9
Redundancy	3.4	3.5	2.8
CC(1/2)		0.998	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	MES (pH=6.5) 50 mM, KCl 200 mM, MPD 35%, CoCl2 10 mM