6M5I

Crystal structure of 2019-nCoV nsp7-nsp8c complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	50.944, 69.376, 101.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.062 - 2.496
R-factor	0.216
Rwork	0.208
R-free	0.29170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f22
Data scaling software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.496	2.500
Number of reflections	6534	892
<I/σ(I)>	8.01
Completeness [%]	99.7
Redundancy	6.85
CC(1/2)	0.998	0.093

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Magnesium formate dihydrate,15% PEG3350.