6M5F

Crystal structure of HinK, a LysR family transcriptional regulator from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97935
Spacegroup name	P 1 21 1
Unit cell lengths	53.534, 152.493, 72.646
Unit cell angles	90.00, 91.80, 90.00

Refinement procedure

Resolution	43.738 - 2.750
R-factor	0.199
Rwork	0.196
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fzv
RMSD bond length	0.005
RMSD bond angle	0.894
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.090	0.047	0.609
Rmeas	0.104	0.054	0.705
Rpim	0.050	0.026	0.347
Total number of observations	116999
Number of reflections	29609	1527	1517
<I/σ(I)>	9.8
Completeness [%]	98.2	98.9	98.9
Redundancy	4	3.9	3.9
CC(1/2)		0.996	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium citrate dibasic, 20% w/v Polyethylene glycol 3350